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| Reyser, Thibaud; To, Tung H.; Egwu, Chinedu; Paloque, Lucie; Nguyen, Michel; Hamouy, Alexandre; Stigliani, Jean-luc; Bijani, Christian; Augereau, Jean-michel; Joly, Jean-patrick; Portela, Julien; Havot, Jeffrey; Marque, Sylvain R. A.; Boissier, Jérôme; Robert, Anne; Benoit-vical, Françoise; Audran, Gérard. |
| Malaria and schistosomiasis are major infectious causes of morbidity and mortality in the tropical and sub-tropical areas. Due to the widespread drug resistance of the parasites, the availability of new efficient and affordable drugs for these endemic pathologies is now a critical public health issue. In this study, we report the design, the synthesis and the preliminary biological evaluation of a series of alkoxyamine derivatives as potential drugs against Plasmodium and Schistosoma parasites. The compounds (RS/SR)-2F, (RR/SS)-2F, and 8F, having IC50 values in nanomolar range against drug-resistant P. falciparum strains, but also five other alkoxyamines, inducing the death of all adult worms of S. mansoni in only 1 h, can be considered as interesting... |
| Tipo: Text |
Palavras-chave: Alkoxyamine; Alkylation; Heme; Malaria; Radical chemistry; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00648/76024/76920.pdf |
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| Crego-vicente, Beatriz; Fernández-soto, Pedro; Febrer-sendra, Begoña; García-bernalt Diego, Juan; Boissier, Jérôme; Angora, Etienne K.; Oleaga, Ana; Muro, Antonio. |
| Schistosomiasis is a disease of great medical and veterinary importance in tropical and subtropical regions caused by different species of parasitic flatworms of the genus Schistosoma. The emergence of natural hybrids of schistosomes indicate the risk of possible infection to humans and their zoonotic potential, specifically for Schistosoma haematobium and S. bovis. Hybrid schistosomes have the potential to replace existing species, generate new resistances, pathologies and extending host ranges. Hybrids may also confuse the serological, molecular and parasitological diagnosis. Currently, LAMP technology based on detection of nucleic acids is used for detection of many agents, including schistosomes. Here, we evaluate our previously developed... |
| Tipo: Text |
Palavras-chave: LAMP; Schistosomiasis; Schistosome hybrids; Schistosoma haematobium; Schistosoma bovis; Molecular diagnosis; Species-specific LAMP; Genus-specific LAMP. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00686/79840/82659.pdf |
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| Rey, Olivier; Toulza, Eve; Chaparro, Cristian; Allienne, Jean-françois; Kincaid-smith, Julien; Mathieu-begné, Eglantine; Allan, Fiona; Rollinson, David; Webster, Bonnie L.; Boissier, Jérôme. |
| Hybridization is a fascinating evolutionary phenomenon that raises the question of how species maintain their integrity. Inter-species hybridization occurs between certain Schistosoma species that can cause important public health and veterinary issues. In particular hybrids between Schistosoma haematobium and S. bovis associated with humans and animals respectively are frequently identified in Africa. Recent genomic evidence indicates that some S. haematobium populations show signatures of genomic introgression from S. bovis. Here, we conducted a genomic comparative study and investigated the genomic relationships between S. haematobium, S. bovis and their hybrids using 19 isolates originating from a wide geographical range over Africa, including samples... |
| Tipo: Text |
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| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00678/79020/81421.pdf |
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| Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier. |
| Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a... |
| Tipo: Text |
Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf |
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| Oleaga, Ana; Rey, Olivier; Polack, Bruno; Grech-angelini, Sébastien; Quilichini, Yann; Pérez-sánchez, Ricardo; Boireau, Pascal; Mulero, Stephen; Brunet, Aimé; Rogon, Anne; Vallée, Isabelle; Kincaid-smith, Julien; Allienne, Jean-françois; Boissier, Jérôme. |
| Environmental and anthropogenic changes are expected to promote emergence and spread of pathogens worldwide. Since 2013, human urogenital schistosomiasis is established in Corsica island (France). Schistosomiasis is a parasitic disease affecting both humans and animals. The parasite involved in the Corsican outbreak is a hybrid form between Schistosoma haematobium, a human parasite, and Schistosoma bovis, a livestock parasite. S. bovis has been detected in Corsican livestock few decades ago raising the questions whether hybridization occurred in Corsica and if animals could behave as a reservoir for the recently established parasite lineage. The latter hypothesis has huge epidemiological outcomes since the emergence of a zoonotic lineage of schistosomes... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00504/61566/65491.pdf |
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| Mulero, Stephen; Toulza, Eve; Loisier, Anaïs; Zimmerman, Meryl; Allienne, Jean-françois; Foata, Joséphine; Quilichini, Yann; Pointier, Jean-pierre; Rey, Olivier; Boissier, Jérôme. |
| To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over... |
| Tipo: Text |
Palavras-chave: Biomonitoring; Corsica; Environmental DNA; Malacology; Metabarcoding; Snail communities. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00666/77777/79917.docx |
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| Kincaid-smith, Julien; Mathieu-bégné, Eglantine; Chaparro, Cristian; Reguera-gomez, Marta; Mulero, Stephen; Allienne, Jean-francois; Toulza, Eve; Boissier, Jérôme; Lamberton, Poppy H. L.. |
| Species usually develop reproductive isolation mechanisms allowing them to avoid interbreeding. These preventive barriers can act before reproduction, “pre-zygotic barriers”, or after reproduction, “post-zygotic barriers”. Pre-zygotic barriers prevent unfavourable mating, while post-zygotic barriers determine the viability and selective success of the hybrid offspring. Hybridization in parasites and the underlying reproductive isolation mechanisms maintaining their genetic integrity have been overlooked. Using an integrated approach this work aims to quantify the relative importance of pre-zygotic barriers in Schistosoma haematobium x S. bovis crosses. These two co-endemic species cause schistosomiasis, one of the major debilitating parasitic diseases... |
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| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00693/80481/83621.pdf |
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| Angora, Etienne K.; Boissier, Jérôme; Menan, Hervé; Rey, Olivier; Tuo, Karim; Touré, Andre O.; Coulibaly, Jean T.; Méité, Aboulaye; Raso, Giovanna; N’goran, Eliézer K.; Utzinger, Jürg; Balmer, Oliver. |
| Schistosomiasis is a parasitic disease affecting more than 250 million people, primarily in sub-Saharan Africa. In Côte d’Ivoire both Schistosoma haematobium (causing urogenital schistosomiasis) and Schistosoma mansoni (causing intestinal schistosomiasis) co-exist. This study aimed to determine the prevalence of S. haematobium and S. mansoni and to identify risk factors among schoolchildren in the western and southern parts of Côte d’Ivoire. From January to April 2018, a cross-sectional study was carried out including 1187 schoolchildren aged 5–14 years. Urine samples were examined by a filtration method to identify and count S. haematobium eggs, while stool samples were subjected to duplicate Kato-Katz thick smears to quantify eggs of S. mansoni and... |
| Tipo: Text |
Palavras-chave: Côte d’Ivoire; Prevalence; Risk factors; Schistosoma haematobium; Schistosoma mansoni; Schistosomiasis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61953/66043.pdf |
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| Guegan, Hélène; Fillaux, Judith; Charpentier, Elena; Robert-gangneux, Florence; Chauvin, Pamela; Guemas, Emilie; Boissier, Jérôme; Valentin, Alexis; Cassaing, Sophie; Gangneux, Jean-pierre; Berry, Antoine; Iriart, Xavier. |
| Background The diagnosis of schistosomiasis currently relies on microscopic detection of schistosome eggs in stool or urine samples and serological assays. The poor sensitivity of standard microscopic procedures performed in routine laboratories, makes molecular detection methods of increasing interest. The aim of the study was to evaluate two in-house real-time Schistosoma PCRs, targeting respectively S. mansoni [Sm] and S. haematobium [Sh] in excreta, biopsies and sera as potential tools to diagnose active infections and to monitor treatment efficacy. Methods Schistosoma PCRs were performed on 412 samples (124 urine, 86 stools, 8 biopsies, 194 sera) from patients with suspected schistosomiasis, before anti-parasitic treatment. Results were compared to... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62534/66865.pdf |
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